By Myrtle A. Davis
Dr. Myrtle A. Davis has assembled a panel of state of the art scientists to explain their most sensible tools for detecting, illuminating, and quantifying apoptotic mechanisms in a fashion that's priceless for the layout of toxicology and pharmacology stories. those state of the art ideas contain stream cytometric, fluorometric, and laser scanning tools for quantifying and characterizing apoptosis, in addition to protocols for using DNA microarray expertise, excessive throughput displays, and ELISA. Immunocytochemical equipment for measuring biochemical and molecular endpoints in tissue sections can be hugely beneficial for these undertaking stories in entire animal types in place of telephone tradition platforms.
Read or Download Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification (Methods in Pharmacology and Toxicology) PDF
Best toxicology books
Delivering a finished overview and replace on contemporary advances, that types a precious reference for practitioners drawn to the newest operative concepts, this cutting-edge textual content stories present and rising issues within the surgical administration of sleep apnea and noisily snoring, inclduing equipment for sufferer overview, avoidance of problems, and postoperative care.
Dietary Toxicology, quantity II, discusses many of the interactions among dietary phenomena and toxicologic strategies. It addresses specific topics that experience develop into considerably extra very important throughout the improvement of latest wisdom, major raises in wisdom, or elevated expertise of capability results on human well-being and health.
This guide offers a large review of the sector, primary realizing of the factitious tools and structure/property correlation, in addition to reports on the topic of functions in quite a lot of topics. The instruction manual additionally presents 1H and 13C NMR spectra, FTIR spectra, DSC and TGA thermograms to assist in examine actions.
Extra resources for Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification (Methods in Pharmacology and Toxicology)
Set up and optimize the flow cytometer as in Basic Protocol 1. UV-excited (360 nm) blue fluorescence (collected with a 450 nm centered bandpass filter) from the Hoechst 33342 dye is proportional with cellular DNA content. To excite MitoTracker Green FM and CMXRosamine stained samples, the argonion laser should be tuned to the 488 nm line. To collect fluorescence from MitoTracker Green FM, use a bandpass filter centered around 530 nm; for CMXRos use a longpass filter above 630 nm (see Notes 5–8).
63. Endresen, P. , Prytz, P. , and Aarbakke, J. (1995) A new flow cytometric method for discrimination of apoptotic cells and detection of their cell cycle specificity through staining of F-actin and DNA. Cytometry 20(2), 162–171. 64. Leers, M. , et al. (1999) Immunocytochemical detection and mapping of a cytokeratin 18 neo- epitope exposed during early apoptosis. J. Pathol. 187(5), 567–572. 65. Morsi, H. , Leers, M. , Kabarity, H. , and Jager, W. (2000) Apoptosis, bcl-2 expression, and proliferation in benign and malignant endometrial epithelium: An approach using multiparameter flow cytometry.
Examples of Detection of Apoptosis with a Confocal Microsocope As with flow cytometry, multiparameter apoptosis assays may also be performed by confocal laser scanning microscopy (CLSM). Using the approach similar to that detailed above for flow cytometry, we have examined NADPH content, mitochondrial membrane potential (CMX Rosamine fluorescence), and mitochondrial mass (Mitotracker Green), by CLSM. Figure 3 shows an example of a typical multiparameter assay performed by confocal microscopy. One advantage to examining apoptotic cells by laser-scanning cytometry is that subcellular structure may be examined.
Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification (Methods in Pharmacology and Toxicology) by Myrtle A. Davis